A Standard Approach to Assessing Disinfection of Environmental Surfaces: Experiments with a Mixture of Surrogates for Infectious Bioagents.
We used a mixture of surrogates (Acinetobacter baumannii, Mycobacterium terrae, hepatitis A virus and spores of Geobacillus stearothermophilus) for bioagents in a standardized approach to test environmental surface disinfectants. Each carrier with 10 mul the mixture received 50 mul of a test chemical or saline at 22+/-2 degrees C. Disinfectant efficacy criteria were >/=6 log10 reduction for the bacteria and the spores and >/=3 log10 for the virus. Peracetic acid (1000 ppm) was effective in 5 min against the two bacteria and the spores but not against the virus. Chlorine dioxide (CD; 500 and 1000 ppm) and domestic bleach (DB; 2500, 3500 and 5000 ppm) were effective in 5 min, except for sporicidal activity which needed 20 min of contact with either 1000 ppm of CD or the two higher conc. of DB. Applied and Environmental Microbiology. 16th July 2010. [Epub ahead of print]. Available at: http://aem.asm.org/cgi/reprint/AEM.00246-10v1.pdf. Accessed 8th February 2011.
Publication Type: Journal article
Publisher: Applied and Environmental Microbiology