One hundred environmental samples were collected in the service sections and public spaces of the reptile department. Sixty-six Salmonella strains were serotyped, 29 belonged to Salmonella enterica ssp. enterica (I), 3 to ssp. salamae (II), 29 to ssp. arizonae or diarizonae (IIIa/b), 4 to ssp. Houtenae (IV) and 1 strain showed autoagglutination. A high prevalence of Salmonella strains was noted in the service sections of the reptile department. Only a few isolations were made in the public spaces. This study confirms the importance of reptiles as spreaders of Salmonella in their surroundings. J Appl Microbiol. 2006;101(2):284-9.