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A carrier test system was designed comprising germination of C. difficile spores for 30 minutes followed by exposure to 70% (v/v) ethanol. A 2-log reduction in germinating C. difficile spores was achieved within 60 minutes. These preliminary results suggest that germination of C. difficile spores increases their susceptibility to 70% ethanol The authors suggest that a two-stage cleaning process comprising germination followed by disinfection may offer an alternative approach to reducing C. difficile spores. Journal of Hospital Infection 2006, 64, supplement 1, P9.01.